AM Paul Davies says Pembrokeshire will be harder hit than any other county in Wales by business rates hikes on the self catering sector.
Pembrokeshire has more than double the number of holiday lets than any other Welsh region and Mr Davies is lobbying for special allowances to be made for the sector.
“Tourism is very important to Pembrokeshire,” he told First Minister Carwyn Jones at the Senedd.
“The introduction of the new business rates next month will have a huge impact on the self catering sector, with many businesses having to pay much more in rates.”
The new bills come into force on April 1 and have to be paid upfront before businesses can appeal.
Figures show that the average bill rise for self catering businesses across Wales is 55% but in Pembrokeshire this rises to 85%.
Mr Davies fears that the higher bills may be the final nail in the coffin for some small operators struggling after two wet summers in a row.
“Everyone is facing tough times at the moment and I fear that these bills will either mean some businesses have to close or put their prices up which will make Wales a less attractive place to visit,” he said.